HPTLC Method Development and Validation: Analytical Method Development and Validation - T. Y. Pasha,B. N. Suhagia,Rajnikant Mardia
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Piegāde 12-18 darba dienu laikā
30 dienu atgriešanas politika
Simultaneous quantification of Lopinavir and Ritonavir in tablet by HPTLC method was developed and validated .The chromatograms were developed using a mobile phase of Chloroform: 1, 4 - Dioxane (7:3 %v/v) on pre-coated plate of silica gel GF aluminum TLC plate and quantified by densitometric absorbance mode at 210 nm. The Rf value for lopinavir and ritonavir was 0.74 and 0.58 respectively. The linearity of ... Pilns apraksts
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Aprašymas
Simultaneous quantification of Lopinavir and Ritonavir in tablet by HPTLC method was developed and validated .The chromatograms were developed using a mobile phase of Chloroform: 1, 4 - Dioxane (7:3 %v/v) on pre-coated plate of silica gel GF aluminum TLC plate and quantified by densitometric absorbance mode at 210 nm. The Rf value for lopinavir and ritonavir was 0.74 and 0.58 respectively. The linearity of the method was found to be within the concentration range of 160-960 ng/spot for Lopinavir and for Ritonavir, it was 40-240 ng/spot. The lower limits of detection and quantification were 9.56 ng/spot and 28.96 ng/spot for Lopinavir and 6.82 ng/spot and 20.66 ng/spot for Ritonavir. The method was also validated for precision, specificity and recovery. This developed method was used to analyze fixed-dose tablet (Lopimune, Cipla Ltd) sample of Lopinavir and Ritonavir.
Vairāk informācijas
| Autors | T. Y. Pasha, B. N. Suhagia, Rajnikant Mardia |
|---|---|
| Izdevējs | LAP Lambert Academic Publishing |
| Izlaides gads | 2012 |
| Vāka tips | Mīkstais vāks |
| EAN | 9783844327359 |